1.细胞发源:vvvv88.com
细胞简称 BT-B
细胞配景态状 These cells were thought to be "stablished from the malign ant urinary bladder carcinoma of a 66-year-old Caucasian m an (stage pTa G3) in 1989"; same donor as for cell line BT-A; however, DNA fingerprinting and cytogenetic analysis at the DSMZ Established unequivocally that this cell line must be considered de facto a subclone of HELA。(STR 检测位点同HELA)
供体年岁 女;30岁6个月
组织起头 膀胱癌组织
细胞面目 上皮细胞样
细胞类型 肿瘤细胞
肿瘤类型 膀胱癌细胞
滋长特点 贴壁细胞
生物安全品级 1vvvv88.com
2.培养及收藏:
竣工培养基 RPMI-1640(PM150110)+10% FBS(164210-50)+1% P/S(PB180120)
培养环境 空气,95%;CO 2,5%
37℃
冻存条目 55% 基础培养基+40